heterogeneity and new epitopes of hepatitis c virus genotype 4
نویسندگان
چکیده
results the present work figured out that the heterogeneity of the quasispecies of our local strains 4a was high showing up 15% diversity. this study also showed four glycosylation sites that play an important role in the entry of the virus and protein folding. besides, different epitpoes were identified in different regions of the e1 and e2 domains; a finding which would help in determining the neutralizing and non- neutralizing antibodies. conclusions this study would help in understanding the driving forces of genetic diversity and would be fundamental for representing potential candidate targets for antibodies and the development of vaccine trials. background hepatitis c virus (hcv) was found to have a major role in human liver disease by its ability to face the host-cell defenses and the immune system. heterogeneity of hcv was the key for its adaptation to its host and represented a significant hurdle for the development of both effective vaccines as well as for novel therapeutic interventions. objectives due to the heterogeneity of hcv virus because of both high replication and high mutation rate in vivo, this study was conducted to analyze different isolates of egyptian patients of genotype 4, of the most mutant regions of the virus (e1 and e2) as they played an important role in viral persistence by escaping from the immune system of the host body. patients and methods this study was conducted through pcr amplification of e1 and e2 regions, sequencing and phylogenetic analysis, calculating synonyms and non-synonyms substitutions, finding the possible glycosylation sites and different epitope domains.
منابع مشابه
Heterogeneity and New Epitopes of Hepatitis C Virus Genotype 4
BACKGROUND Hepatitis C virus (HCV) was found to have a major role in human liver disease by its ability to face the host-cell defenses and the immune system. Heterogeneity of HCV was the key for its adaptation to its host and represented a significant hurdle for the development of both effective vaccines as well as for novel therapeutic interventions. OBJECTIVES Due to the heterogeneity of HC...
متن کاملNS5A Sequence Heterogeneity and Mechanisms of Daclatasvir Resistance in Hepatitis C Virus Genotype 4 Infection
BACKGROUND Daclatasvir is an NS5A inhibitor approved for treatment of infection due to hepatitis C virus (HCV) genotypes (GTs) 1-4. To support daclatasvir use in HCV genotype 4 infection, we examined a diverse genotype 4-infected population for HCV genotype 4 subtype prevalence, NS5A polymorphisms at residues associated with daclatasvir resistance (positions 28, 30, 31, or 93), and their effect...
متن کاملHeterogeneity of hepatitis C virus genotype 4 strains circulating in south-western France.
Hepatitis C virus (HCV) is a major cause of liver disease. Knowledge of HCV variability is crucial for clinical and epidemiological analysis. HCV genotype 4 (HCV-4) has become increasingly prevalent in European countries, including France, in recent years. The present study investigates the heterogeneity of HCV-4 in south-western France by phylogenetic analysis of NS5B sequences from 166 patien...
متن کاملHepatitis C virus genotype 4 therapy: progress and challenges.
The hepatitis C virus genotype 4 (HCV-4) is prevalent in Egypt, the Middle East and Africa. Recently, the epidemiology of HCV-4 has changed and this genotype has begun to cross borders and spread to several regions in Europe through immigration and injection drug use. HCV-4 has been considered a difficult-to-treat genotype based on the low sustained virological response (SVR) rates obtained wit...
متن کاملAntibodies Targeting Novel Neutralizing Epitopes of Hepatitis C Virus Glycoprotein Preclude Genotype 2 Virus Infection
Currently, there is no effective vaccine to prevent hepatitis C virus (HCV) infection, partly due to our insufficient understanding of the virus glycoprotein immunology. Most neutralizing antibodies (nAbs) were identified using glycoprotein immunogens, such as recombinant E1E2, HCV pseudoparticles or cell culture derived HCV. However, the fact that in the HCV acute infection phase, only a small...
متن کاملHepatitis C virus and skin
Nowadays viral hepatitis is one of the most common and important causes of chronic liver disorders in the world. Extrahepatic manifestations of viral hepatitis consist a main part of the disease. The main viruses causing hepatitis include Hepatitis A, B and C viruses. Herein common skin manifestations in hepatitis C virus (HCV) are discussed. Chronic hepatitis causes skin disorders both directl...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
hepatitis monthlyجلد ۱۳، شماره ۸، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023